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Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose BIOMOLECULES, v.11, no.2, 2021-02
SCOPUS 14

Keywords Synechocystis 7338 exogenous glucose photosynthetic pigments metabolic profiling lipidomic profiling GC-MS nanoESI-MS

Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose
Noh, YuJin ; Lee, Hwanhui ; Kim, Myeongsun ; Hong, Seong-Joo ; Lee, Hookeun ; Kim, Dong-Myung ; Cho, Byung-Kwan ; Lee, Choul-Gyun ; Choi, Hyung-Kyoon

초록	Synechocystis strains are cyanobacteria that can produce useful biomaterials for biofuel and pharmaceutical resources. In this study, the effects of exogenous glucose (5-mM) on cell growth, photosynthetic pigments, metabolites, and lipids in Synechocystis sp. PCC 7338 (referred to as Synechocystis 7338) were investigated. Exogenous glucose increased cell growth on days 9 and 18. The highest production (mg/L) of chlorophyll a (34.66), phycocyanin (84.94), allophycocyanin (34.28), and phycoerythrin (6.90) was observed on day 18 in Synechocystis 7338 culture under 5-mM glucose. Alterations in metabolic and lipidomic profiles under 5-mM glucose were investigated using gas chromatography-mass spectrometry (MS) and nanoelectrospray ionization-MS. The highest production (relative intensity/L) of aspartic acid, glutamic acid, glycerol-3-phosphate, linolenic acid, monogalactosyldiacylglycerol (MGDG) 16:0/18:1, MGDG 16:0/20:2, MGDG 18:1/18:2, neophytadiene, oleic acid, phosphatidylglycerol (PG) 16:0/16:0, and PG 16:0/17:2 was achieved on day 9. The highest production of pyroglutamic acid and sucrose was observed on day 18. We suggest that the addition of exogenous glucose to Synechocystis 7338 culture could be an efficient strategy for improving growth of cells and production of photosynthetic pigments, metabolites, and intact lipid species for industrial applications.
서지사항	BIOMOLECULES, v.11, no.2, 2021-02
ISSN	2218-273X
주제분야	Biochemistry & Molecular Biology
저자 키워드	Synechocystis 7338 exogenous glucose photosynthetic pigments metabolic profiling lipidomic profiling GC-MS nanoESI-MS
DOI	10.3390/biom11020214
자료유형	Article
기관	KAIST

Comparative Primary Metabolic and Lipidomic Profiling of Freshwater and Marine Synechocystis Strains Using by GC-MS and NanoESI-MS Analyses BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.25, no.2, pp.308 - 319, 2020-03
SCOPUS 12 KCI 11

Keywords metabolic profiling lipidomic profiling freshwater Synechocystis 6803 marine Synechocystis 7338 GC-MS nanoESI-MS

Comparative Primary Metabolic and Lipidomic Profiling of Freshwater and Marine Synechocystis Strains Using by GC-MS and NanoESI-MS Analyses
Noh, YuJin ; Lee, Hwanhui ; Hong, Seong-Joo ; Lee, Hookeun ; Cho, Byung-Kwan ; Lee, Choul-Gyun ; Choi, Hyung-Kyoon

초록	Synechocystis strains are considered as valuable resources for production of useful metabolites. Comparative primary metabolic and lipidomic profiling of freshwater and marine Synechocystis strains is needed to understand the physiological characteristics of each strain at molecular level. In this study, we performed comparative primary metabolic and lipidomic profiling of freshwater Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) and marine Synechocystis sp. PCC 7338 (hereafter Synechocystis 7338) by using gas chromatography-mass spectrometry and nano electrospray ionization-mass spectrometry. Neophytadiene, phytol, monogalactosyldiacylglycerol (MGDG), and digalactosyldiacylglycerol (DGDG) were significantly higher in Synechocystis 6803. Levels of alanine, aspartic acid, valine, linoleic acid, linolenic acid, oleic acid, plamitic acid, stearic acid, glucosylglycerol, sucrose, phosphatidylglycerol (PG), and diacylglyceryltrimethylhomoserine (DGTS) (only detected in Synechocystis 7338) were significantly higher in Synechocystis 7338. Our results provide basic information to choose Synechocystis strains for various industrial usages, and also provide fundamental information on the future development of novel Synechocystis strains to produce high levels of specific metabolites by metabolic pathway modulation.
서지사항	BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.25, no.2, pp.308 - 319, 2020-03
ISSN	1226-8372
주제분야	Biotechnology & Applied Microbiology
저자 키워드	metabolic profiling lipidomic profiling freshwater Synechocystis 6803 marine Synechocystis 7338 GC-MS nanoESI-MS
DOI	10.1007/s12257-019-0432-8
자료유형	Article
기관	KAIST

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